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chip grade anti trim33  (Bethyl)


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    Structured Review

    Bethyl chip grade anti trim33
    Chip Grade Anti Trim33, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+trim33+antibody/TRIM33%2FTIF1gamma+Antibody/pm41731944-180-19-22
    Average 93 stars, based on 23 article reviews
    chip grade anti trim33 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Trim33 Binds and Silences a Class of Young Endogenous Retroviruses in the Mouse Testis; a Novel Component of the Arms Race between Retrotransposons and the Host Genome
    Article Snippet: .. To identify the binding sites of Trim33, across the genome, the same anti-Trim33 antibody (Bethyl Laboratories A301-060A) was used to perform ChIP in adult testis followed by high throughput sequencing of Trim33-bound DNA. ..

    Chromatin Immunoprecipitation:

    Article Title: Trim33 Binds and Silences a Class of Young Endogenous Retroviruses in the Mouse Testis; a Novel Component of the Arms Race between Retrotransposons and the Host Genome
    Article Snippet: .. To identify the binding sites of Trim33, across the genome, the same anti-Trim33 antibody (Bethyl Laboratories A301-060A) was used to perform ChIP in adult testis followed by high throughput sequencing of Trim33-bound DNA. ..

    Article Title: Trim33 mediates the proinflammatory function of Th17 cells
    Article Snippet: .. ChIP assays were done as described ( ) with anti-Trim33 antibody (A301-059A/060A; Bethyl Laboratories), anti-H3K9/14Ac (06–599; Upstate), anti-H3K4me antibody (07-473; Upstate), anti-H3K9me3 antibody (ab8898; Abcam), anti-H3K27me3 (07-449; Upstate) antibody, or control IgG. ..

    Article Title: Trim33 is required for appropriate development of pre-cardiogenic mesoderm
    Article Snippet: Control and mutant EBs were fixed cross-linked with 1% formaldehyde for 30 min, washed and sonicated on ice for 8 min (1s on - 1s off sonication cycle; 50% power output) using the Sonic Ruptor 250 sonicator equipped with a microtip (Omni International). .. Chromatin immunoprecipitation using ChIP-qualified anti-Trim33 antibody (Bethyl A301– 060A) was carried out using the SimpleChIP Plus Sonication ChIP kit (#56383 Cell Signaling Technology) following the manufacturer’s instructions. qPCR reactions were carried out using the Applied Biosystems ViiA7 real-time PCR system and Power SYBR Green PCR master mix (Thermo Fisher 4367659). ..

    Article Title: Macrophage production and activation are dependent on TRIM33
    Article Snippet: .. ChIP experiments were carried out with an anti-TRIM33 antibody (A301-059A, Bethyl) as previously described [ ]. .. For sequencing, 10ng of purified DNA from ChIP was adapter-ligated, PCR amplified and sequenced by Illumina Genome Analyzer IIx according to the manufacturer's instructions (Illumina).

    Next-Generation Sequencing:

    Article Title: Trim33 Binds and Silences a Class of Young Endogenous Retroviruses in the Mouse Testis; a Novel Component of the Arms Race between Retrotransposons and the Host Genome
    Article Snippet: .. To identify the binding sites of Trim33, across the genome, the same anti-Trim33 antibody (Bethyl Laboratories A301-060A) was used to perform ChIP in adult testis followed by high throughput sequencing of Trim33-bound DNA. ..

    Control:

    Article Title: Trim33 mediates the proinflammatory function of Th17 cells
    Article Snippet: .. ChIP assays were done as described ( ) with anti-Trim33 antibody (A301-059A/060A; Bethyl Laboratories), anti-H3K9/14Ac (06–599; Upstate), anti-H3K4me antibody (07-473; Upstate), anti-H3K9me3 antibody (ab8898; Abcam), anti-H3K27me3 (07-449; Upstate) antibody, or control IgG. ..

    Article Title: TRIM33 plays a critical role in regulating dendritic cell differentiation and homeostasis by modulating Irf8 and Bcl2l11 transcription
    Article Snippet: Lin – FLT3 + cells isolated from the BM of wild-type C57BL/6 mice were lysed in Western and IP lysis buffer (Beyotime, #P0013) supplemented with proteinase and phosphatase inhibitors on ice for 30 min. .. The lysates were incubated with an anti-TRIM33 antibody (Bethyl Laboratories, #A301-059A) or control IgG (Cell Signaling Technology, #2729) at 4 °C overnight before the immunoprecipitates were purified with Protein A + G magnetic beads (Millipore, #16-663). .. The samples were then subjected to SDS‒PAGE for mass spectrometry analysis at the Technology Center for Protein Sciences of Tsinghua University.

    Article Title: TRIM33 plays a critical role in regulating dendritic cell differentiation and homeostasis by modulating Irf8 and Bcl2l11 transcription.
    Article Snippet: Lin–FLT3+ cells isolated from the BM of wild-type C57BL/6 mice were lysed in Western and IP lysis buffer (Beyotime, #P0013) supplemented with proteinase and phosphatase inhibitors on ice for 30min. .. The lysates were incubated with an anti-TRIM33 antibody (Bethyl Laboratories, #A301-059A) or control IgG (Cell Signaling Technology, #2729) at 4 °C overnight before the immunoprecipitates were purified with Protein A+G magnetic beads (Millipore, #16-663). .. The samples were then subjected to SDS‒PAGE for mass spectrometry analysis at the Technology Center for Protein Sciences of Tsinghua University.

    Sonication:

    Article Title: Trim33 is required for appropriate development of pre-cardiogenic mesoderm
    Article Snippet: Control and mutant EBs were fixed cross-linked with 1% formaldehyde for 30 min, washed and sonicated on ice for 8 min (1s on - 1s off sonication cycle; 50% power output) using the Sonic Ruptor 250 sonicator equipped with a microtip (Omni International). .. Chromatin immunoprecipitation using ChIP-qualified anti-Trim33 antibody (Bethyl A301– 060A) was carried out using the SimpleChIP Plus Sonication ChIP kit (#56383 Cell Signaling Technology) following the manufacturer’s instructions. qPCR reactions were carried out using the Applied Biosystems ViiA7 real-time PCR system and Power SYBR Green PCR master mix (Thermo Fisher 4367659). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Trim33 is required for appropriate development of pre-cardiogenic mesoderm
    Article Snippet: Control and mutant EBs were fixed cross-linked with 1% formaldehyde for 30 min, washed and sonicated on ice for 8 min (1s on - 1s off sonication cycle; 50% power output) using the Sonic Ruptor 250 sonicator equipped with a microtip (Omni International). .. Chromatin immunoprecipitation using ChIP-qualified anti-Trim33 antibody (Bethyl A301– 060A) was carried out using the SimpleChIP Plus Sonication ChIP kit (#56383 Cell Signaling Technology) following the manufacturer’s instructions. qPCR reactions were carried out using the Applied Biosystems ViiA7 real-time PCR system and Power SYBR Green PCR master mix (Thermo Fisher 4367659). ..

    SYBR Green Assay:

    Article Title: Trim33 is required for appropriate development of pre-cardiogenic mesoderm
    Article Snippet: Control and mutant EBs were fixed cross-linked with 1% formaldehyde for 30 min, washed and sonicated on ice for 8 min (1s on - 1s off sonication cycle; 50% power output) using the Sonic Ruptor 250 sonicator equipped with a microtip (Omni International). .. Chromatin immunoprecipitation using ChIP-qualified anti-Trim33 antibody (Bethyl A301– 060A) was carried out using the SimpleChIP Plus Sonication ChIP kit (#56383 Cell Signaling Technology) following the manufacturer’s instructions. qPCR reactions were carried out using the Applied Biosystems ViiA7 real-time PCR system and Power SYBR Green PCR master mix (Thermo Fisher 4367659). ..

    Polymerase Chain Reaction:

    Article Title: Trim33 is required for appropriate development of pre-cardiogenic mesoderm
    Article Snippet: Control and mutant EBs were fixed cross-linked with 1% formaldehyde for 30 min, washed and sonicated on ice for 8 min (1s on - 1s off sonication cycle; 50% power output) using the Sonic Ruptor 250 sonicator equipped with a microtip (Omni International). .. Chromatin immunoprecipitation using ChIP-qualified anti-Trim33 antibody (Bethyl A301– 060A) was carried out using the SimpleChIP Plus Sonication ChIP kit (#56383 Cell Signaling Technology) following the manufacturer’s instructions. qPCR reactions were carried out using the Applied Biosystems ViiA7 real-time PCR system and Power SYBR Green PCR master mix (Thermo Fisher 4367659). ..

    other:

    Article Title: The transcriptional cofactor TRIM33 prevents apoptosis in B lymphoblastic leukemia by deactivating a single enhancer
    Article Snippet: Anti-TRIM33 antibody (A301-060A; Bethyl Laboratories, Montgomery, TX), Anti-PU.1 antibody (#2266; Cell Signaling, Beverly, MA or sc-352; Santa Cruz, Dallas, TX), Anti-Bim antibody (#2819; Cell Signaling), Anti-H3K27ac antibody (ab4729; Abcam, Cambridge, MA), Anti-H3K4me3 antibody (07-473; Millipore), Anti-ß-actin HRP antibody (#A3854; Sigma, Ronkonkoma, NY), APC anti-mouse B220 (#103212; BioLegend, San Diego, CA), APC anti-mouse CD-19, APC anti-mouse Mac-1/Cd11b (#101211; BioLegend), APC anti-mouse Ly-6G/Gr-1 (#17-5931; eBioscience, San Diego, CA), APC anti-mouse TER-119 (#116212; BioLegend), APC anti-mouse CD-3 (#100209; BioLegend).

    Incubation:

    Article Title: TRIM33 plays a critical role in regulating dendritic cell differentiation and homeostasis by modulating Irf8 and Bcl2l11 transcription
    Article Snippet: Lin – FLT3 + cells isolated from the BM of wild-type C57BL/6 mice were lysed in Western and IP lysis buffer (Beyotime, #P0013) supplemented with proteinase and phosphatase inhibitors on ice for 30 min. .. The lysates were incubated with an anti-TRIM33 antibody (Bethyl Laboratories, #A301-059A) or control IgG (Cell Signaling Technology, #2729) at 4 °C overnight before the immunoprecipitates were purified with Protein A + G magnetic beads (Millipore, #16-663). .. The samples were then subjected to SDS‒PAGE for mass spectrometry analysis at the Technology Center for Protein Sciences of Tsinghua University.

    Article Title: TRIM33 plays a critical role in regulating dendritic cell differentiation and homeostasis by modulating Irf8 and Bcl2l11 transcription.
    Article Snippet: Lin–FLT3+ cells isolated from the BM of wild-type C57BL/6 mice were lysed in Western and IP lysis buffer (Beyotime, #P0013) supplemented with proteinase and phosphatase inhibitors on ice for 30min. .. The lysates were incubated with an anti-TRIM33 antibody (Bethyl Laboratories, #A301-059A) or control IgG (Cell Signaling Technology, #2729) at 4 °C overnight before the immunoprecipitates were purified with Protein A+G magnetic beads (Millipore, #16-663). .. The samples were then subjected to SDS‒PAGE for mass spectrometry analysis at the Technology Center for Protein Sciences of Tsinghua University.

    Purification:

    Article Title: TRIM33 plays a critical role in regulating dendritic cell differentiation and homeostasis by modulating Irf8 and Bcl2l11 transcription
    Article Snippet: Lin – FLT3 + cells isolated from the BM of wild-type C57BL/6 mice were lysed in Western and IP lysis buffer (Beyotime, #P0013) supplemented with proteinase and phosphatase inhibitors on ice for 30 min. .. The lysates were incubated with an anti-TRIM33 antibody (Bethyl Laboratories, #A301-059A) or control IgG (Cell Signaling Technology, #2729) at 4 °C overnight before the immunoprecipitates were purified with Protein A + G magnetic beads (Millipore, #16-663). .. The samples were then subjected to SDS‒PAGE for mass spectrometry analysis at the Technology Center for Protein Sciences of Tsinghua University.

    Article Title: TRIM33 plays a critical role in regulating dendritic cell differentiation and homeostasis by modulating Irf8 and Bcl2l11 transcription.
    Article Snippet: Lin–FLT3+ cells isolated from the BM of wild-type C57BL/6 mice were lysed in Western and IP lysis buffer (Beyotime, #P0013) supplemented with proteinase and phosphatase inhibitors on ice for 30min. .. The lysates were incubated with an anti-TRIM33 antibody (Bethyl Laboratories, #A301-059A) or control IgG (Cell Signaling Technology, #2729) at 4 °C overnight before the immunoprecipitates were purified with Protein A+G magnetic beads (Millipore, #16-663). .. The samples were then subjected to SDS‒PAGE for mass spectrometry analysis at the Technology Center for Protein Sciences of Tsinghua University.

    Magnetic Beads:

    Article Title: TRIM33 plays a critical role in regulating dendritic cell differentiation and homeostasis by modulating Irf8 and Bcl2l11 transcription
    Article Snippet: Lin – FLT3 + cells isolated from the BM of wild-type C57BL/6 mice were lysed in Western and IP lysis buffer (Beyotime, #P0013) supplemented with proteinase and phosphatase inhibitors on ice for 30 min. .. The lysates were incubated with an anti-TRIM33 antibody (Bethyl Laboratories, #A301-059A) or control IgG (Cell Signaling Technology, #2729) at 4 °C overnight before the immunoprecipitates were purified with Protein A + G magnetic beads (Millipore, #16-663). .. The samples were then subjected to SDS‒PAGE for mass spectrometry analysis at the Technology Center for Protein Sciences of Tsinghua University.

    Article Title: TRIM33 plays a critical role in regulating dendritic cell differentiation and homeostasis by modulating Irf8 and Bcl2l11 transcription.
    Article Snippet: Lin–FLT3+ cells isolated from the BM of wild-type C57BL/6 mice were lysed in Western and IP lysis buffer (Beyotime, #P0013) supplemented with proteinase and phosphatase inhibitors on ice for 30min. .. The lysates were incubated with an anti-TRIM33 antibody (Bethyl Laboratories, #A301-059A) or control IgG (Cell Signaling Technology, #2729) at 4 °C overnight before the immunoprecipitates were purified with Protein A+G magnetic beads (Millipore, #16-663). .. The samples were then subjected to SDS‒PAGE for mass spectrometry analysis at the Technology Center for Protein Sciences of Tsinghua University.



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    Image Search Results


    Development and validation of novel monoclonal antibodies to the ecto domain of TrIP. A , cloning strategy and development of the ecto-TrIP Fc-fusion protein used for immunization and antibody development. B , SDS-PAGE of the ectoTrIP-Ig fusion protein under reducing and nonreducing conditions. C , flow cytometry data showing the binding of each anti-TrIP monoclonal antibody to a Flag-tagged WT murine TrIP construct expressed in HEK293T cells. D , flow cytometry data showing staining of the clones on HEK293T cells transfected with a plasmid encoding Flag-tagged human TrIP. E , flow cytometry data showing the staining of each of the anti-TrIP mAbs on HEK293T cells transfected with a Flag-tagged mTrIP construct lacking the extracellular kringle domain (ΔKringle). HEK, human embryonic kidney; mAb, monoclonal antibody; mTrIP, murine TrIP; TrIP, transmembrane inhibitor of PI3K.

    Journal: The Journal of Biological Chemistry

    Article Title: Downregulation of PIK3IP1/TrIP on T cells is controlled by TCR signal strength, PKC, and metalloprotease-mediated cleavage

    doi: 10.1016/j.jbc.2024.107930

    Figure Lengend Snippet: Development and validation of novel monoclonal antibodies to the ecto domain of TrIP. A , cloning strategy and development of the ecto-TrIP Fc-fusion protein used for immunization and antibody development. B , SDS-PAGE of the ectoTrIP-Ig fusion protein under reducing and nonreducing conditions. C , flow cytometry data showing the binding of each anti-TrIP monoclonal antibody to a Flag-tagged WT murine TrIP construct expressed in HEK293T cells. D , flow cytometry data showing staining of the clones on HEK293T cells transfected with a plasmid encoding Flag-tagged human TrIP. E , flow cytometry data showing the staining of each of the anti-TrIP mAbs on HEK293T cells transfected with a Flag-tagged mTrIP construct lacking the extracellular kringle domain (ΔKringle). HEK, human embryonic kidney; mAb, monoclonal antibody; mTrIP, murine TrIP; TrIP, transmembrane inhibitor of PI3K.

    Article Snippet: Monoclonal antibodies to the ecto domain of murine TrIP were developed in conjunction with a commercial vendor (Rockland, Inc).

    Techniques: Biomarker Discovery, Bioprocessing, Cloning, SDS Page, Flow Cytometry, Binding Assay, Construct, Staining, Clone Assay, Transfection, Plasmid Preparation